Population Density of the Florida Scrub Lizard (\u3cem\u3eSceloporus woodi\u3c/em\u3e) in Managed Sand Pine Scrub and Longleaf Pine Sandhill Habitats

Studies investigating managed landscapes are of increasing importance, as fragmentation is a known cause of biodiversity loss. From June to September 2012, we sampled populations of the rare, endemic Florida Scrub Lizard (Sceloporus woodi) across the Ocala National Forest (ONF) to compare lizard density across two managed habitat types. Florida Scrub habitat in the ONF is clearcut and roller-chopped, whereas Longleaf Pine habitat is managed via prescribed burning. We sampled 10 stands of Florida Scrub (2–3 y post disturbance) and 10 stands of Longleaf Pine (1 y post-disturbance) for lizards. We compared lizard density between the interior of each habitat patch and the associated natural surface road habitat surrounding each habitat patch. To compare microhabitat conditions between stand types, we also gathered vegetation and substrate data. Lizards occurred in higher density in recently burned Longleaf Pine than in roller-chopped scrub. Stands of roller-chopped scrub showed a noticeable absence of lizards. Higher lizard density along roads suggests that lizards use natural surface roads extensively. Scrub and longleaf stands differed in several microhabitat conditions, which may be related to differences in density. Further research is needed to examine the effects of disturbance frequency, patch size, and isolation on the overall persistence of the Florida Scrub Lizard population in the ONF

Forelimb Position Affects Facultative Bipedal Locomotion in Lizards

Recent work indicates that bipedal posture in lizards is advantageous during obstacle negotiation. However, little is known about how bipedalism occurs beyond a lizard\u27s acceleratory threshold. Furthermore, no study to date has examined the effects of forelimb position on the body center of mass (BCoM) in the context of bipedalism. This study quantified the frequency of bipedalism when sprinting with versus without an obstacle at 0.8 m from the start of a sprint. Forelimb positions were quantified during bipedal running at the start of a sprint and when crossing an obstacle. Two species with contrasting body forms (and thus different BCoM) were studied (Sceloporus woodi and Aspidoscelis sexlineata) to assess potential variation due to body plan and obstacle-crossing behavior. No significant difference in frequency of bipedalism was observed in S. woodi with or without an obstacle. However, A. sexlineata primarily used a bipedal posture when sprinting. Forelimb positions were variable in S. woodi and stereotyped in A. sexlineata. Caudal extension of the forelimbs helped shift the BCoM posteriorly and transition to, or maintain, a bipedal posture in A. sexlineata, but not in S. woodi. The posterior shift in BCoM, aided by more caudally placed forelimbs, helps raise the trunk from the ground, regardless of obstacle presence. The body plan, specifically the length of the trunk and tail, and forelimb position work together with acceleration to shift the BCoM posteriorly to transition to a bipedal posture. Thus, species exhibit morphological and behavioral adjustments to transition to and maintain facultative bipedalism while sprinting

Getting Up to Speed: Acceleration Strategies in the Florida Scrub Lizard, \u3cem\u3eSceloporus woodi\u3c/em\u3e

Small animals typically rely on quick bursts and intermittent pauses when moving in the wild. Hence, the study of acceleration capacity is important for understanding the ecology and evolution of locomotor performance. In this study, we investigate intraspecific variation in the acceleration capacity of a small lizard (Sceloporus woodi). To quantify animal acceleration performance, the momentum‐impulse theorem is applied to data collected from high‐speed video recordings of individuals accelerating from a standstill and over a subsequent distance of 0.4 m. Unlike earlier studies, the momentum‐impulse approach allows one to directly and precisely quantify the per step contribution to acceleration capacity. Like other small vertebrates, we show that S. woodi is capable of accelerating to near maximum speeds (∼2 m s−1) within ∼0.4 m and needs only a few steps (at least five) to achieve maximum speed. However, considerable intraspecific variation in acceleration capacity exists; individuals take different numbers of steps (two to five steps) over the first 0.4 m, and only some individuals (10 of 19) reach their maximum speed over the first 0.4 m. Only acceleration performance in steps 1 and 2 is predictive of running speed at 0.4 m; accelerations in steps 3, 4, and 5 are not related to individual differences in speed. Individual variation in acceleration strategy is considerable, with individuals using one of three strategies to reach maximum speed. Muscle mass‐specific power during acceleration approaches the maximum power output measured for lizard hindlimb musculature (∼900 W kg−1), suggesting that S. woodi accelerations approach the limit of their musculoskeletal system. This study highlights the utility of the momentum‐impulse approach to study acceleration performance and the importance of elucidating the per step contribution to acceleration capacity

Towards a unified theory of intensional logic programming

AbstractIntensional Logic Programming is a new form of logic programming based on intensional logic and possible worlds semantics. Intensional logic allows us to use logic programming to specify nonterminating computations and to capture the dynamic aspects of certain problems in a natural and problem-oriented style. The meanings of formulas of an intensional first-order language are given according to intensional interpretations and to elements of a set of possible worlds. Neighborhood semantics is employed as an abstract formulation of the denotations of intensional operators. Then we investigate general properties of intensional operators such as universality, monotonicity, finitariness and conjunctivity. These properties are used as constraints on intensional logic programming systems. The model-theoretic and fixpoint semantics of intensional logic programs are developed in terms of least (minimum) intensional Herbrand models. We show in particular that our results apply to a number of intensional logic programming languages such as Chronolog proposed by Wadge and Templog by Abadi and Manna. We consider some elementary extensions to the theory and show that intensional logic program clauses can be used to define new intensional operators. Intensional logic programs with intensional operator definitions are regarded as metatheories

Allometry, sexual size dimorphism, and niche partitioning in the Mediterranean gecko (Hemidactylus turcicus)

Hemidactylus tucrius is a small gekkonid lizard native to the Middle East and Asia that is known to exhibit sexual dimorphism in head size. Several potential explanations exist for the evolution and maintenance of sexual dimorphism in lizards. We tested 2 of these competing hypotheses concerning diet partitioning and differential growth. We found no differences in Average meal size (volume) or in any single dimension of prey size for similarly sized males and females. allometric patterns of increases in head size also were measured in males and females. We found that males exhibited a mixture of isometric and positively allometric patterns of head size increases, whereas females exhibited isometric and negatively allometric patterns. Thus, we concluded that sexual dimorphism in head size is not the result of diet partitioning but instead of differential growth patterns following sexual maturity in males and females

Use of Trees by the Texas Ratsnake (\u3cem\u3eElaphe obsoleta\u3c/em\u3e) in Eastern Texas

We present information on the use of trees by Elaphe obsoleta (Texas Ratsnake) in a mesic pine-hardwood forest in eastern Texas. Using radiotelemetry, seven snakes (3 females, 4 males) were relocated a total of 363 times from April 2004 to May 2005, resulting in 201 unique locations. Snakes selected trees containing cavities and used hardwoods and snags for a combined 95% of arboreal locations. Texas Ratsnake arboreal activity peaked during July and August, well after the peak of avian breeding activity, suggesting arboreal activity involves factors other than avian predation

Use of Trees by the Texas Ratsnake (Elaphe obsoleta) in Eastern Texas

We present information on the use of trees by Elaphe obsoleta (Texas Ratsnake) in a mesic pine-hardwood forest in eastern Texas. Using radiotelemetry, seven snakes (3 females, 4 males) were relocated a total of 363 times from April 2004 to May 2005, resulting in 201 unique locations. Snakes selected trees containing cavities and used hardwoods and snags for a combined 95% of arboreal locations. Texas Ratsnake arboreal activity peaked during July and August, well after the peak of avian breeding activity, suggesting arboreal activity involves factors other than avian predation

Effects of Training and Testosterone on Muscle-Fiber Types and Locomotor Performance in Male Six-Lined Racerunners (\u3cem\u3eAspidoscelis sexlineata\u3c/em\u3e)

Testosterone (T) is thought to affect a variety of traits important for fitness, including coloration, the size of sexual ornaments, aggression, and locomotor performance. Here, we investigated the effects of experimentally elevated T and locomotor training on muscle physiology and running performance in a nonterritorial male lizard species (Aspidoscelis sexlineata). Additionally, several morphological attributes were quantified to examine other characters that are likely affected by T and/or a training regimen. Neither training alone nor training with T supplementation resulted in increased locomotor performance. Instead, we found that T and training resulted in a decrease in each of three locomotor performance variables as well as in hematocrit, ventral coloration, and testis size. Strikingly, neither the size nor the fiber composition of the iliofibularis or gastrocnemius muscles was different among the two treatments or a group of untrained control animals. Hence, the relationships among T, training, and associated characters are not clear. Our results offer important insights for those hoping to conduct laboratory manipulations on nonmodel organisms and highlight the challenges of studying both training effects and the effects of steroid hormones on locomotor performance

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Linking Patterns of Locomotor Behavior & Performance to Predator Avoidance and the Ecology of a Small Reptile

This talk was given to Georgia College & State University